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In most cases, you should not use a pruning seal on trees after trimming. Applying a sealant can interfere with the tree's natural healing process, and it does not guarantee protection from pathogens. Instead of sealing the cut, the best practice is to support the tree's natural recovery by providing it with plenty of water and a dormant-style fertilizer. However, there are exceptions. Pruning seals may be recommended for very vulnerable species, like elm trees, in areas where dangerous diseases such as Dutch elm disease are prevalent. In these specific situations, the protection offered by a sealant can outweigh its downsides. For advice tailored to your situation, especially after cutting large branches, it is best to consult a local tree trimming professional.

Bleach can potentially kill some types of delicate ornamental trees, but for the most part, it will only kill the foliage it comes in contact with. Applying bleach is not an effective method of killing a tree. A better option is to use an herbicide, which is a chemical compound designed to effectively kill a tree. 

If you’re planning on keeping the tree, unless the roots are invasive, there’s no need to remove them, as doing so can cause damage to the tree. If the tree has been cut down and the stump and roots are left, grinding and removing the stump can cause root decay for some species. For other species, the stump and roots will need to be removed to prevent pests, disease, and possible tree regrowth.

If a disease has attacked and destroyed the roots, then the tree may fall over as soon as it has died or while part of it is still alive. Trees that die with their roots left in place tend to stay standing for much longer, but those roots will decay eventually, allowing gravity to do the rest.