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Yes, weeds will grow through pea gravel but you can prevent this during the installation process. Install a weed barrier underneath pea gravel and swap it out every three years to keep weeds at bay.

Mowing your grass regularly, especially if you’re mowing close to the ground, can help kill Johnsongrass. Frequent mowing helps limit the spread of Johnsongrass and can even deplete carbohydrate reserves in rhizomes over time. The more time you leave in between mowing, the more likely Johnsongrass is to regrow and take over your lawn. 

No, it's not really possible to completely prevent weed growth. Weeds are incredibly resilient—all they usually need is a tiny bit of dirt to sprout, so their eventual appearance is pretty much inevitable. However, you can definitely keep them at bay by getting rid of weeds as soon as you notice them. The longer you let them sit and grow roots, the more they'll spread.

Yellow leaves and stems on your sprouts indicate they’re not getting enough light. Keep them near the appropriate light source and give them as much as they need (they require longer exposure to fluorescence than they do to natural light sources).