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Once the animals and their young are out of the house, remove the nest and locate any entry points. Some may be difficult to find because some animals can fit through very small holes. Once you’ve located as many entry points as possible, use materials such as metal or masonry to block off the entry. Ideally, you should choose materials that most animals cannot chew through. Create a one-way door or flap to allow animals to leave but not return. Additional methods include using repellants designed to make an area unappealing to the types of animals that find your home so inviting. 

Squirrels hate not having access to secure shelter or food sources. Your best line of defense is to make your property less attractive by removing access to these things. There are chemical squirrel repellents, but these have mixed success. According to Pennsylvania State University, you can apply products containing the bittering agent denatonium saccharide to a squirrel's favorite fence, tree, or roof spots. However, you need to reapply repellents regularly, which aren’t scientifically proven effective.

It’s debatable, but most will say either termites or bed bugs are the hardest to remove. When dealing with termites, you must find the central nest (as opposed to satellite nests) and exterminate the queen and all potential queens, which often takes the work of a specialist. Bed bugs, meanwhile, are incredibly hardy, good at hiding, and resistant to pesticides, so it takes very careful, very thorough work to get rid of them permanently. 

Identifying the specific animal running on your roof at night can be challenging without direct observation. Common nocturnal roof visitors include raccoons, squirrels, opossums, and rats. Raccoons are bigger and can create heavy thumping sounds. Squirrels are agile and may produce rapid scurrying noises. Opossums tend to move stealthily with less noise. Rats can make scratching or gnawing sounds. To determine the culprit, look for signs like footprints, droppings, or damaged areas near the roof. For accurate identification, consult a pest control professional or set up wildlife cameras near potential entry points.