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Weeds can grow through rubber mulch, but it depends on if you removed all the weeds in your garden beforehand. Weeds already in the soil can grow even when using rubber mulch. However, that doesn’t mean that your garden will be littered with rubber and weeds, but it does mean that the occasional weed might still pop up. Another way you can increase your weed suppression is by using landscape fabric as a second layer. 

Yes, organic herbicides and natural prevention methods reduce chemical use.

No, it's not really possible to completely prevent weed growth. Weeds are incredibly resilient—all they usually need is a tiny bit of dirt to sprout, so their eventual appearance is pretty much inevitable. However, you can definitely keep them at bay by getting rid of weeds as soon as you notice them. The longer you let them sit and grow roots, the more they'll spread.

Vinegar is known to be a contact herbicide, which means it kills the foliage it comes in contact with. Because of the high acetic acid concentration, horticultural vinegar kills weeds and grass more effectively. However, keep in mind that high amounts and multiple applications can affect the soil by lowering the pH levels.