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Both spikes and loose nets can be installed over nest sites to prevent the bird from returning, but nets are not a humane option since birds and other wildlife can get tangled in them. 

Spikes are a humane alternative that deter nesting without harming the birds. In small areas, like vents, a very fine mesh net can be installed to prevent the bird from returning. These nets don't generally pose a risk to the birds.

Regular cleaning, proper food storage, sealing entry points, and professional inspections help prevent infestations.

You can find a variety of humane bird repellent sprays online and at home improvement stores. However, if you want to try a DIY method that will keep birds away without harming them, mix some water, dish soap, and a few drops of peppermint oil and spray the area you want bird-free. Peppermint oil is a notoriously lovely smell for humans, but birds tend to avoid it.

Identifying the specific animal running on your roof at night can be challenging without direct observation. Common nocturnal roof visitors include raccoons, squirrels, opossums, and rats. Raccoons are bigger and can create heavy thumping sounds. Squirrels are agile and may produce rapid scurrying noises. Opossums tend to move stealthily with less noise. Rats can make scratching or gnawing sounds. To determine the culprit, look for signs like footprints, droppings, or damaged areas near the roof. For accurate identification, consult a pest control professional or set up wildlife cameras near potential entry points.