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DIY treatments exist, but professional extermination is recommended for thorough elimination and prevention.

There is no exact science here, due to the nature of nests and how mice raise their young, but a good place to start is to inspect the droppings. A single mouse poops a lot, leaving around 50 droppings in a single day, likely near a primary food source. If you find only sporadic droppings, you likely have a small infestation. If you spot large mounds of droppings, you are dealing with a big problem.

Spiders are not typically attracted to bedding as a primary source of food or shelter, but they sometimes inadvertently end up there. Spiders can hitch a ride on your clothing, belongings, or through open windows and doors. The underside of a bed, especially if it's near a wall or corner, can provide such a sheltered spot where they can hide and build webs. Dust and clutter under your bed can create ideal hiding places as well. If your room has other insects like flies or mosquitoes, spiders may be drawn to the area in search of prey. 

Homes provide a stable environment with ample hiding spots, warmth, and protection from the elements, making them ideal habitats for spiders. The presence of insects within homes serves as a major food source for spiders, enticing them to establish their webs or burrows in and around residential structures. The nooks and crannies of homes provide perfect locations for spiders to construct webs or lay in wait for unsuspecting prey.