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Both spikes and loose nets can be installed over nest sites to prevent the bird from returning, but nets are not a humane option since birds and other wildlife can get tangled in them. 

Spikes are a humane alternative that deter nesting without harming the birds. In small areas, like vents, a very fine mesh net can be installed to prevent the bird from returning. These nets don't generally pose a risk to the birds.

The best animal repellents for gardens include a variety of different scents. You can use predator urine to keep away deer, raccoons, squirrels, and more. The odor will tell the critters that your yard is a dangerous place. Other scents that repel critters are herbs like lavender, geranium, and mint. You can also use hot pepper, garlic, and strong-smelling soap.

DIY animal removal is rarely a good idea and is not advised for many reasons. First, these types of animals can carry diseases and, therefore, pose a health risk to you, your family, and your pets if mishandled. Second, depending on your city or municipality, it may be illegal to remove or handle wildlife yourself. 

Sealing your home against these animals yourself is also a bad idea. Trapping an animal under your house will cause odor and insect problems when it passes away. 

Identifying the specific animal running on your roof at night can be challenging without direct observation. Common nocturnal roof visitors include raccoons, squirrels, opossums, and rats. Raccoons are bigger and can create heavy thumping sounds. Squirrels are agile and may produce rapid scurrying noises. Opossums tend to move stealthily with less noise. Rats can make scratching or gnawing sounds. To determine the culprit, look for signs like footprints, droppings, or damaged areas near the roof. For accurate identification, consult a pest control professional or set up wildlife cameras near potential entry points.