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There should be a 1-inch thick layer of sand underneath your pavers in order to provide a proper base.

Using sand under stepping stones can be beneficial in certain situations. Sand can help level the ground and provide stability for the stepping stones, especially if the ground is uneven. It also allows for minor adjustments during the installation process. However, sand may not be the best choice for all environments. In areas with high foot traffic or heavy rain, sand may wash away or become displaced, leading to an uneven surface. In such cases, a more stable base material like gravel or crushed stone is preferable. Consider your specific landscape conditions before deciding whether to use sand under stepping stones.

If you like the look of mulch in your plant beds, be aware that it can attract a variety of pests, including termites. To avoid wood-to-foundation contact, leave a one-foot barrier around your house and fill it in with rocks or gravel, starting mulch past that barrier and working it outward into your plant beds. 

While you don’t need to put anything down before mulch, you can apply a pre-emergent herbicide to prevent weed growth. As long as you remove weeds before you spread mulch, you should be in the clear. But a herbicide is something that you can put down before mulch as an extra layer of protection.