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Weeds can grow through rubber mulch, but it depends on if you removed all the weeds in your garden beforehand. Weeds already in the soil can grow even when using rubber mulch. However, that doesn’t mean that your garden will be littered with rubber and weeds, but it does mean that the occasional weed might still pop up. Another way you can increase your weed suppression is by using landscape fabric as a second layer. 

If possible, you should prioritize pulling weeds up by hand. However, be sure not to use your bare hands, as many weeds are sharp or otherwise irritating when they come in contact with skin. Always don thick, protective gardening gloves to protect your hands when you pull up weeds.

Vinegar is known to be a contact herbicide, which means it kills the foliage it comes in contact with. Because of the high acetic acid concentration, horticultural vinegar kills weeds and grass more effectively. However, keep in mind that high amounts and multiple applications can affect the soil by lowering the pH levels.

Herbicide application, manual removal, and landscape fabric are common approaches.